Isolation, Characterization and Phylogenetic Analysis of the Cytochrome B Gene (Cyb) from the Hypotrichous Ciliate Pseudourostyla cristata
نویسندگان
چکیده
To date, no genes have been characterized from mitochondrial DNA of hypotrichous ciliates. Here we present the complete sequence of the gene (cyb) encoding cytochrome b in the hypotrichous ciliate Pseudourostyla cristata. Analysis of nucleotide and deduced amino acids sequences predicts a protein of 393 amino acids encoded by cyb. CYB amino acid similarity and phylogenetic tree analyses strongly support a close evolutionary relationship between Paramecium, Tetrahymena and Pseudourostyla. The heat stress-dependent expression pattern of CYB was verified by semi-quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis.
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